Molecular Microbial Ecology and Genomics Laboratory
The Molecular Microbial Ecology and Genomics Lab is fully equipped for state-of-the-art molecular biology, genomics, and bioinformatics research including capabilities to facilitate high-throughput molecular biology work (multichannel pipettors, 96-sample gel apparatus, vacuum apparatus for 96-well plate mini-prepping, purifications), cultivation and recombinant DNA research.
The laboratory is fully equipped with the instrumentation, supporting equipment and computational resources required for closing and annotating genome sequences.
The lab also has thermal cyclers, an ABI 3130 Avant Genetic Analyzer (for DNA sequencing and fragment analysis), refrigerated and room temperature microfuges, a Nanodrop spectrophotometer, hybridization oven, several gel electrophoresis boxes, a DGGE unit, and UV transilluminator and digital imaging system (Kodak). The lab is set up to perform DNA microarray hybridizations, including a microfluidic hybridization station, staining dishes and racks for array manipulations, hybridization cassettes, water baths, hot blocks, centrifuge with microtiter plate spinning capacity, and an Axon 4000B Scanner. A suite of microbial growth rooms (-10°C and 4°C), shaking and stationary incubators in addition to storage facilities including -80oC and -20°C freezers, and 4°C chromatography refrigerator are also available. A Milli-Q water system is in place providing RNAse-free water. Expected completion of a new 4500 sq.ft. wing will be added to the Maxey Building at DRI to accommodate a new laboratory being custom-designed for Murray’s group. The primary lab will be 660 sq ft. with three shared equipment rooms totaling ~300 sq ft.
Adjacent shared-use equipment include a Varian spectrophotometer, Molecular Dynamics 96-well spectrometer and a 96-well fluorometer, Chef DR-II pulsed field gel electrophoresis (BioRad), laminar flow hood, autoclave, and Olympus BX60 Epifluorescence Microscope with MTI CCD camera and image-Pro Plus image analysis software. In Summer 2007 a new Confocal Microscope will be acquired with research funds from a collaborative NSF-MRI grant.
Dr. Murray leads a group studying the molecular biological (DNA/RNA/Protein) make‐up and biogeochemical impacts of microorganisms living in oceanic and terrestrial polar environments, in association with marine invertebrates, in alpine snowpack, polar and desert hypersaline systems and even the deep sea. Common themes across this research are studying the diversity of life using gene, (meta)genome and metatranscriptome approaches, and studying the biogeochemical processes occurring and capabilities required for survival under varying conditions and environmental extremes.
Dr. Alison Murray
Diversity, ecology and evolution of archaea and bacteria, focus on marine systems, polar microbial ecosystems, environmental microbial genomics, specifically, functional and comparative genomics, astrobiology, life detection methods
Dr. Zoe Harrold
Geochemistry, Geomicrobiology and Astrobiology
Biotechnology MS, UNR
Fullbright Graduate Fellow
Marine Biology, Ph.D. Univ. Hawaii
Biotechnology BS/MS Program, UNR
Postgraduate training intern
Chemistry, UNR, 2010
Dr. Joseph Grzymski (2004-2007)
Dr. Christian Riesenfeld (2006-2009)
Alexandra Kameda (Environmental Sciences and Health, M.S., UNR, Dec. 2006)
Annika Mosier (Environmental Resource Sciences, M.S., UNR, Dec. 2004)
Graduate students (committee members)
Dr. Sara Nassar (Computer Science and Engineering, UNR; Ph.D. May 2008)
Dr. Kendra Zamzow (Environmental Sciences and Health, UNR. Ph.D. Dec. 2007)
Dr. Mihailo Kaplarevic (Computer Engineering, Ph.D., U. Delaware, Apr 2007)
Nipesh Shah (Computer Science, M.S. UNR, Dec., 2004)
Sarah Marshall (Environmental Sciences and Health, M.S., UNR, July, 2002)
Dr. Claribel Cruz-Garcia (Soil Sciences, Ph.D. MSU Aug. 2002)
Alex Dussaq (Biochemistry and Molecular Biology, UNR Fall 2006 – Spring 2008; INBRE Summer research Fellowship Summer 2007, and NSF EPSCOR Undergraduate Research Fellowship 07-08 academic year)
Scott Lucas (Biology, UNR, Fall 2004 through Spring 2006)
Devin Currie (UM Amherst, Summer 2005)
Jason Michaels (Biochemistry, UNR, Spring 2003)
Alexandra Kameda (Biology, UNR Summer-Fall 2002)
High School students:
Alex Dussaq (Summer 2006)
Kaitlyn Shakleton (Summer 2005)
David Shakleton (Summer 2003)