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Study provides new insight into how microbes process nitrogen

David Vuono

Reno, Nev. (February 20, 2019): Microbes play a key role in Earth’s nitrogen cycle, helping to transform nitrogen gas from the atmosphere back and forth into organic forms of nitrogen that can be used by plants and animals.

New research from the Desert Research Institute in Reno, Nev. provides new insight into how this process happens, through the examination of a unique species of microbe called Intrasporangium calvum that was found in a contaminated groundwater well at Oak Ridge National Laboratory Field Research Station in Tennessee.

The study, which published in Frontiers in Microbiology in January, examined the response of I. calvum to different concentrations of environmental resources and how those differences impacted the microbe’s nitrogen cycling ability. The study team also investigated the evolution of this microbe, the biochemistry behind the reactions, and how each of those factors interact with the environment.

Although most microbes perform just one step in the nitrogen cycle – converting nitrogen gas (N2) from the atmosphere to ammonia (NH3) in the soil, for example – the research team discovered that I. calvum could perform two types of reactions: respiratory ammonification and denitrification. Respiratory ammonification retains nitrogen in an ecosystem as ammonium in the soil or water, while denitrification sends nitrogen on a path back to the atmosphere as a gas.

“The microbe that we studied is unique because it can essentially ‘breathe’ in nitrogen and then send the nitrogen along one of two pathways, ‘exhaling’ either ammonium or nitrous oxide,” said David Vuono, Ph.D., postdoctoral researcher fellow with DRI’s Division of Earth and Ecosystem Sciences and Applied Innovation Center, and lead author of the new study. “This is kind of like humans breathing in oxygen and then having the ability to exhale either carbon dioxide or methane.”

021319 Vuono 2Sample bottles of I. calvum are sterilized via flame in the Laboratory of Molecular Responses at DRI.

With the ability to perform more than one type of reaction – either sending nitrogen back to the atmosphere or retaining it in the soil or water – Vuono and his team wondered what would trigger the microbe to select one pathway versus the other. Previous studies had concluded that the ratio of carbon (C) to nitrate (NO3-) in the surrounding environment was the determining factor, but Vuono wondered if the story wasn’t actually more complex.

In this study, Vuono and his team looked beyond the C:NO3- ratio to investigate the importance of the overall concentration of each nutrient. They tested the response of I. calvum under conditions of both high and low resource availability, while keeping the ratio of C:NO3- at a constant level.

According to their findings, it is the resource concentration, rather than the C:NO3- ratio, that determines pathway selection. When grown under low carbon concentrations, the team found that these microbes were more likely to process nitrogen by ammonification; under high carbon concentrations, denitrification prevailed.

“As we learned, the concentration of nutrients available to these microbes is what determines where the nitrogen ends up, whether it takes a pathway back towards the atmosphere or returns to ammonium,” Vuono explained. “That is a really important distinction, because depending on the environment that you’re in, you may want to remove nitrogen or you may want to retain it.”

In a waterway, for example, high levels of nitrogen can cause algae blooms and dead zones; by creating conditions that favor denitrification, it is possible that microbes could be triggered to send nitrogen back to the atmosphere. In an agricultural field, on the other hand, nitrogen deficiencies in the soil can lead to poor plant growth; by creating conditions that would promote respiratory ammonification, microbes could be prompted to retain nitrogen in the soils, eliminating or lessening the need for chemical fertilizers.

021319 Vuono 3David Vuono prepares a sample of I.calvum for analysis.

Although this study took place under laboratory conditions, Vuono is now planning a follow-up study on microbial communities found in nature. He hopes that his research provides a new perspective on what might be regulating metabolic pathways in other organisms in the environment, and why.

“Our findings are important because they challenge a long-held belief that the ratio of carbon to nitrogen drives pathway selection, and will help researchers to better predict nutrient cycling in environments where oxygen is in support supply,” Vuono said.

This study was funded by the Nevada Governor’s Office of Economic Development (GOED), the Desert Research Institute postdoctoral research fellowship program, Ecosystems and Networks Integrated with Genes and Molecular Assemblies (ENIGMA), and Oak Ridge National Laboratory (US Department of Energy, Office of Science, Office of Biological and Environmental Research).

Other DRI scientists who contributed to this study included Robert Read, John A. Arnone III, Iva Neveux, Evan Loney, David Miceli, and Joseph Grzymski. The full study, titled Resource Concentration Modulates the Fate of Dissimilated Nitrogen in a Dual-Pathway Actinobacterium, is available online from Frontiers in Microbiology (22 January, 2019): https://doi.org/10.3389/fmicb.2019.00003

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The Desert Research Institute (DRI) is a recognized world leader in basic and applied interdisciplinary research. Committed to scientific excellence and integrity, DRI faculty, students, and staff have developed scientific knowledge and innovative technologies in research projects around the globe. Since 1959, DRI's research has advanced scientific knowledge, supported Nevada's diversifying economy, provided science-based educational opportunities, and informed policy makers, business leaders, and community members. With campuses in Reno and Las Vegas, DRI is one of eight institutions in the Nevada System of Higher Education.

 

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